Minimum irradiance required to inhibit microbial growth to prevent ventilatorassociated pneumonia by an endotracheal tube equipped with blue LEDs

Artificial respiration is saving lives especially in the COVID-19 pandemic, but it also carries the risk to cause ventilator-Associated pneumonia (VAP). VAP is one of the most common and severe nosocomial infections, often leading to death and adding a major economic burden to the healthcare system. To prevent a proliferation of microbial pathogens that cause VAP, an endotracheal tube (ETT) equipped with blue LEDs (LED-ETT) was developed. This blue wavelength exhibits antimicrobial properties but may also harm human tracheal cells at higher irradiances. Therefore, the aim of this study was to find the minimal required irradiance for microbial reduction of 1 log level in 24 h by applying LED-ETTs. A LED-ETT with 48 blue LEDs (450 nm) was fixed in a glass tube, which served as a trachea model. The investigation was carried out with irradiations of 4.2, 6.6 and 13.4 mW/cm² at 37 °C for 24 h. The experiments were performed with Acinetobacter kookii as a surrogate of Acinetobacter baumannii, which is classified as critical by the WHO. Samples of A. kookii suspensions were taken every 4 h during irradiation from the trachea model. Bacteria concentrations were quantified by determining colony forming units (CFU)/ml. A homogeneous irradiance of only 4.2 mW/cm² generated by the blue LEDs, at a LED forward current of 3.125 mA, is sufficient to achieve a 1 log reduction of A. kookii within 24 h. The total irradiation dose within this period was 360 J/cm2. Human cells survive this dose without cellular damage. Previous studies revealed that the pathogen A. baumannii is even more sensitive to blue light than A. kookii. Therefore, blue LED-ETTs are expected to reduce A. baumannii without harming human tracheal cells. © 2021 by Walter de Gruyter Berlin/Boston.

Minimum irradiance required to inhibit microbial growth to prevent ventilator-associated pneumonia by an endotracheal tube equipped with blue LEDs

Introduction Artificial respiration is saving lives especially in the COVID-19 pandemic, but it also contains the risk to cause ventilatorassociated pneumonia (VAP). VAP is one of the most common and severe nosocomial infections often leading to death and adding a major economic burden to the healthcare system. To prevent a proliferation of microbial pathogens that cause VAPs, an endotracheal tube equipped with blue LEDs (LED-ETT) was developed. This blue wavelength exhibits antimicrobial properties but may also harm human tracheal cells. Therefore, the aim of this paper was to find the minimal required irradiance for microbial reduction of 1 log level in 24 h by applying LED-ETTs. Methods A LED-ETT with 48 blue LEDs (450 nm) was fixated in a glass tube, which functions as a trachea model. The investigation was carried out with irradiations of 4.2, 6.6 and 13.4 mW/cm2 at 37 °C for 24 h. The experiments were performed with Acinetobacter kookii as a surrogate of Acinetobacter baumannii, which is classified as critical by the WHO. Samples of A. kookii suspensions were taken every 4h during irradiation from the trachea model. Bacteria were quantified by determining colony forming units cfu/ml. Results A homogeneous irradiance of only 4.2 mW/cm2 generated by the blue LEDs, at a current of 25 mA, is sufficient to achieve a 1 log reduction of A. kookii within 24 h. The total irradiation dose within this period was 360 J/cm2. Conclusion 360 J/cm2is a dose that human cells survive without cellular damage. Previous studies revealed that the pathogen A. baumannii is even more sensitive to blue light. Therefore, blue LED-ETTs are expected to reduce A. baumannii without harming human tracheal cells.